Understanding a protein’s amino acid sequence is key to elucidating its structure and function. Edman degradation is a powerful technique for sequentially determining a protein’s amino acid sequence from the N-terminus.
This analytical method was developed by Pehr Edman in the 1950s and remains a useful tool for protein sequencing today.
Here’s how it works:
The peptide or protein chain is reacted under mild conditions to label and cleave off the amino acid at the N-terminus in the first cycle. The truncated peptide is then reacted again to remove the next residue, and so on for many cycles.
At each step, the derivatized amino acid is identified, revealing the sequence from the N-terminus sequentially.
Key Advantages of Edman Degradation:
- Directly determines amino acid sequence from protein’s N-terminus
- Can be applied to peptides and proteins with up to 60 amino acid residues
- More accurate than mass spectrometry for sequencing short chains
- Able to identify modified or unusual amino acids
Edman Degradation In Action:
See the technique in action in this step-by-step illustration of sequencing a hypothetical tetrapeptide:
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Cycle 1: Alanine Glycine Valine Leucine
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Cycle 2: Glycine Valine Leucine
Cycle 3: Valine Leucine
Cycle 4: Leucine
Learn More About Protein Sequencing
Edman degradation has played an important role in helping uncover the sequences and structures of proteins central to biology and disease research.
To learn more about this classic protein sequencing technique, its applications, and modern variations, contact our experts today.
Chemistry provides a framework for industry, technology, and research. It establishes the fundamentals of the biological sciences. The goal of the chemistry course is to provide pupils a broad understanding of daily life. It will help students understand how things are made and how each element is chemically put together. A confluence of all these factors makes chemistry a highly challenging subject for the majority of pupils. Nonetheless, the writers for writersabc will make any assignment clear-cut and easy to understand. Below is a sample of a question.
Draw the expected Edman degradation product (a PTH derivative) of Lys-Gly-Met-Ala after one cycle. What is the peptide obtained after this cycle?
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